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KMID : 0380619850170040295
Korean Journal of Food Science and Technology
1985 Volume.17 No. 4 p.295 ~ p.303
Detection of Aflatoxins in Soybean Food by HPLC


Abstract
Aflatoxin B©û, B©ü, G©û, and G©ü were quantitatively detected by the high pressure liquid chromatography on a Micropak-CN column, with Hexane-THF-IPA-water, using a Lichrosorbpacked flowceil in the fluorometric detector. Under those conditions, the minimum detectable amount of aflatoxin B©û was 0.2 ng. HPLC was used in determining amount of aflatoxins in the commercially manufactured soybean food and home-made Meju. Aflatoxin producing abilities of strains used in the industrially fermented soybean food were also studied with the HPLC technique. Although aflatoxin-like substances were detected in a few samples on TLC, they were not identified with the HPLC retention times of standard aflatoxins. The commercial fungal strains used in Korea had no aflatoxin producing abilities.
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